Aim of the webinar: To provide participants with an overview on possible recovery and purification options, as required on the basis of product characteristics, feedstock type and other relevant process condition and/or limitations (cost, flexibility, sustainability). A brief introduction to biochemical engineering with particular reference to separation processes. New trends in bioprocess integration and intensification. Primary recovery. This will include commonly utilised operations like centrifugation and filtration (dead-end and tangential). Utilisation of membranes as a tool for fractionation. Cell disruption processes when product is located in the intracellular space. Biomass pre-treatment for raw materials. Product Purification. Adsorption and Chromatography. Application of adsorptive technology a) to gain selectivity during membrane operations and b) to “integrate” operations by direct capture. Additional opportunities for downstream process integration: Aqueous-Two partitioning. Partitioning of proteins and system design. Principles of size-exclusion chromatography as polishing step. Advantages and limitations.