A flexible fermentation pilot with 3 fermentors 30, 300 and 3000 litres; aerobic, anaerobic, batch, fed batch and continuous. Cellulosic sugar hydrolysates produced on site by enzymatic hydrolysis.
Type of Collaboration:Fee paying basisFunded collaborative projectJoint ventures
What "non-technical" services relevant to scale-up does your organisation offer?:None
ASSET INFORMATION
Asset Information:
A flexible fermentation pilot with 3 fermentors 30, 300 and 3000 litres; aerobic, anaerobic, batch, fed batch and continuous. Cellulosic sugar hydrolysates produced on site by enzymatic hydrolysis.
Enzymatic hydrolysis (for supply of sugars, not for experimenting), Fermentation - GMO, Evaporation, centrifuge, decanter, washing, ultrafiltration, fully instrumented. 3 fermentors ,
Borregaard operators must run the process. Tenants will only be allowed to supervise the experiments.
Tenants responsible for test procedure, taking care of products and analyzing results
24h operation, 5 days a week
Possible to run fermentation over weekend in case no operators needed
Tenants will receive raw data from the control system on a data file
Hydrolysis reactors will be used by Borregaard to produce glucose from cellulose (hydrolysates). Cellulose substrate for hydrolysis will be unbleached sulphite pulp. Tenants may supply their own enzymes for hydrolysis of cellulose and/or tenants may also choose to supply their own sugar substrate for fermentation.
3 Bioreactors/fermentors, GMO approved, 30 L, 300 L, 3000 L
Control of pH, temperature oxygen, foam, pressure and level.
Air/N2 flow
Measurement of CO2/O2 in gas out
Seed train from agar to 2 litres scale in lab part of the service.
Batch, fed batch and continuous fermentations
Separate feeding of each reactor
Feeding directly from feedstock tanks, no preblending needed, giving flexibility
Decanter, 3140 G, 5300 rpm, 150-1500 l/h
Centrifuge, clarification, 250 l/h
Rod filter, 40µm, semi-continuous filtration
2 x Bag filter, >5µm
Disc filter, >0,2µm
Plenty of storage capacity (tanks)
Vacuum evaporator feeding capacity 150-620 kg/h
Flash evaporator feeding capacity 0-300 kg/h
Ultrafiltration rig can be made available. Tenants will have to purchase the (PCI?) tubular membranes of their choice.
10 bar, Hidrostal Pump
10-50 bar, Ram Pump